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Subhrangsu S. Mandal |
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Biochemistry
Assistant Professor B.Sc. (Honors) Midnapore College, India (1989)M.Sc. (First Class First) Kalyani University, India (1992) Ph.D. Indian Institute of Science (1998) Postdoctoral Fellow: |
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Phone: 817-272-3804 FAX: 817-272-3808 E-mail: smandal@uta.edu Office: 349 CPB Personal Page |
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Research interests: Transcription
is a process by which the genetic information in DNA gets converted into
RNA. In eukaryotes, the key
enzyme involved in transcription of most of the protein coding genes is
called RNA polymerase II (RNA pol II). In
eukaryotes, genes are compacted into nucleosome, the repeating unit of
chromatin that is composed of histones and DNA. Recent
studies showed that post-transcriptional modifications of histones (e.g.,
acetylation, phosphorylation, methylation, ubiquitilation, etc.) mark and
dictate whether a specific gene will be turned “on or off” and is also
associated with various human cancer. My research
goal is to understand “How RNA pol II transcribes a gene under chromatin
and non-chromatin environment? What
are the functions of various covalent histone modifications in chromatin
transcription, gene expression, epigenetic regulation and cancer in
human?” More specifically,
we study: (I) Identification
and characterization of novel transcription factors involved in efficient
transcription by RNA pol II through chromatin: Highly purified
reconstituted chromatin transcription system under cell free environment
are used primarily for these experiments. (II)
Roles of histone methyltransferases Set1, MLL1 and MLL2 in gene
expression; MLL mediated
oncogenesis (acute myeloid or lymphoid Leukemia) in human; Epigenetics,
histone modification and chromatin transcription. (III) Mechanism of eukaryotic transcription by RNA polymerase II (fluorescence spectroscopy with 2-aminopurine as probe in vitro, FRET analysis in vivo). Representative Publications: Zhu B., Mandal S. S., Pham A. D., Zheng Y., Erdjument-Bromage H., Batra
S. K., Tempst P., and Reinberg D. (2005) "The
human PAF complex coordinates transcription with events downstream of RNA
synthesis." Genes Dev.,
19, 1668-73. Mandal
S. S., Chun C., Wada T., Handa H., Shatkin A. and Reinberg D. "Functional
interactions between mRNA capping enzyme and factors that positively and
negatively regulate transcription during promoter escape." Proc. Natl. Acad. Sci. (USA) 2004, 101,
7572-77. Mandal
S. S., Sims R. J.
and Reinberg D. (2004) Review
article on "Recent highlights in RNAP II mediated
transcription." Current Opinion in Cell Biology, 16, 263-71. Endou M., Hasegawa J., Watanabe
H., Kim D., Inukai N., Narita T., Shima D., Yamada T., Furuya A., Sato H.,
Hasegawa M., Yamaguchi Y., Mandal
S. S., Reinberg D., Wada T. and Handa H. (2004) "Human SPT6 stimulates transcription elongation by RNA
polymerase II in vitro." Mol. Cell. Biol., 24, 3324-36. Chen, B., Mandal S. S. and Hampsey, M. (2004) "Mechanism of transcription start site selection by
transcription factor TFIIB. A
photo-crosslinking study." Biochemistry,
43, 12741-49. Mandal S. S., Cho H., Kim S.,
Cabane K. and Reinberg D. (2002) "FCP1,
a phosphatase specific for the heptapeptide repeat of the largest subunit
of RNA polymerase II, stimulates transcription elongation." Mol.
Cell. Biol., 22, 7543-52. Mandal S. S., Silva E. F. and
Reha-Krantz, L. J. (2002) "Using
2-aminopurine fluorescence to detect base unstacking in the template
strand during nucleotide incorporation by the bacteriophage T4 DNA
polymerase." Biochemistry,
41(13), 4399-4406. Fidalgo Da Silva E., Mandal S. S. and Reha-Krantz, L. J. (2002) "Using 2-aminopurine fluorescence to measure incorporation of incorrect nucleotides by the bacteriophage T4 DNA polymerase: Evidence for a rate-determining step before chemistry." J. Biol. Chem., 277, 40640-49.
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Department of Chemistry and Biochemistry |
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